| days | 日付 | |
| 1 | defreeze feeder cells | |
| 2 | ||
| 3 | defreeze feeder cells | |
| 4 | make feeder layer (60 mm dish) | |
| 5 | defreeze ES cells | |
| 6 | refeed (ESM) | |
| 7 | passage ES cells / make feeder layer (60 mm dish) | |
| 8 | electroporation | |
| 9 | refeed (ESM) | |
| 10 | refeed (G418) / defreeze feeder cells | |
| 11 | refeed (G418) | |
| 12 | refeed (G418) | |
| 13 | refeed (G418) | |
| 14 | ||
| 15 | refeed (G418) / make feeder layer (96 well dish) | |
| 16 | pick up ES colonies 1 | |
| 17 | refeed (ESM) | |
| 18 |
freeze ES cells / subculture to gelatin-coated 24 well dish pick up ES colonies 2 |
|
| 19 | refeed (ESM) | |
| 20 | ||
| 21 | freeze ES cells / subculture to gelatin-coated 24 well dishextract genomic DNA of ES cells | |
| 22 | Southern blot |